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Vector Backbone Description: Backbone Size:12095; Vector Backbone:pET28a and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_122001 Copy
Vector Backbone Description: Backbone Size:16599; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Comments: Please note that the plasmid is prone to recombination between the duplicate tac promoter-lac operator. Addgene recommends that you test 2-3 colonies via digest or sequencing to confirm that the full 16.6kb plasmid was selected.
Proper citation: RRID:Addgene_121998 Copy
Species: E. coli
Genetic Insert: aac3(IV)
Vector Backbone Description: Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Apramycin
Defining Citation: PMID:12923110
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_68112 Copy
Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110094 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110091 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, CRISPR, i; Bacterial Resistance:Apramycin
Defining Citation: PMID:29862648
Proper citation: RRID:Addgene_110185 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, CRISPR, i; Bacterial Resistance:Apramycin
Defining Citation: PMID:29862648
Proper citation: RRID:Addgene_110183 Copy
Vector Backbone Description: Backbone Size:5927; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126597 Copy
Species: Other
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34083482
Proper citation: RRID:Addgene_174518 Copy
Vector Backbone Description: Vector Backbone:The plasmid is built using multiple fragments; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:33608525
Proper citation: RRID:Addgene_174655 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Comments: Please visit https://www.biorxiv.org/content/10.1101/582403v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125689 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117231 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5634
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167451 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5056
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167368 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5238
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167401 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5053
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167367 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5048
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167366 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5248
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167405 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5044
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167364 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5262
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167409 Copy
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