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Vector Backbone Description: Backbone Size:12095; Vector Backbone:pET28a and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_122001 Copy
Vector Backbone Description: Backbone Size:16599; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548010
Comments: Please note that the plasmid is prone to recombination between the duplicate tac promoter-lac operator. Addgene recommends that you test 2-3 colonies via digest or sequencing to confirm that the full 16.6kb plasmid was selected.
Proper citation: RRID:Addgene_121998 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117231 Copy
Vector Backbone Description: Vector Backbone:pCOND; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110094 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110091 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, CRISPR, i; Bacterial Resistance:Apramycin
Defining Citation: PMID:29862648
Proper citation: RRID:Addgene_110185 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, CRISPR, i; Bacterial Resistance:Apramycin
Defining Citation: PMID:29862648
Proper citation: RRID:Addgene_110183 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Comments: Please visit https://www.biorxiv.org/content/10.1101/582403v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125689 Copy
Vector Backbone Description: Vector Backbone:pBeloBAC11; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:31611640
Proper citation: RRID:Addgene_158211 Copy
Genetic Insert: LuxABCDE
Vector Backbone Description: Vector Backbone:pApr_2L; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:31611640
Proper citation: RRID:Addgene_158210 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0156
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159032 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0150
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159030 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0152
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159031 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0034
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_158999 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0317
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159009 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0248
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159007 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0252
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159008 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0035
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159000 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0615
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159017 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0005
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159027 Copy
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