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Genetic Insert: sgRNA scaffold
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:2880; Vector Backbone:PX551-Plasmid #60957; Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30231914
Proper citation: RRID:Addgene_112139 Copy
Species: Homo sapiens
Genetic Insert: PRM-5R
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6600; Vector Backbone:pMal-tev; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22398450
Proper citation: RRID:Addgene_112088 Copy
Species: Homo sapiens
Genetic Insert: Abl-PRM-4R
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6600; Vector Backbone:pMal-tev; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22398450
Proper citation: RRID:Addgene_112087 Copy
Species: Homo sapiens
Genetic Insert: KRAB
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_112195 Copy
Species: Other
Genetic Insert: Super-folder GFP
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700229
Proper citation: RRID:Addgene_112225 Copy
Species: Mus musculus
Genetic Insert: (Arkadia)Rnf111
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pTriEx2-hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17341133
Comments: The GFP tagged Arkadia constructs were generated by eliminating the first 9 aa of Arkadia and fusing in frame to GFP Sequences and cloned into SmaI/XhoI of the pTriEx2-hygro (Novagen, http://www.novagen.com) vector.
Proper citation: RRID:Addgene_112228 Copy
Species: Ideonella sakaiensis
Genetic Insert: PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
Vector Backbone Description: Backbone Marker:Novagen/Millipore Sigma; Backbone Size:5367; Vector Backbone:pET-21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29666242
Proper citation: RRID:Addgene_112202 Copy
Species: Homo sapiens
Genetic Insert: EYFP-YAP1
Vector Backbone Description: Backbone Size:7484; Vector Backbone:pLentilox3.7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909276
Comments: The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)
Proper citation: RRID:Addgene_112289 Copy
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid # 48015); Vector Backbone:pAGM4723; Vector Types:Golden Gate assembly; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37278403
Comments: L2 empty backbone for golden gate assembly (Bpi) to diatoms.
This is a shorter backbone to improve transformation and cloning for systems that do not need the Agrobacterium T-DNA delivery.
The colonies are red due to the canthaxanthin operon. Successful insertion cut out the canthaxanthin operon and lead to white colonies.
Proper citation: RRID:Addgene_112207 Copy
Species: Synthetic
Genetic Insert: SpyCatcher-mi3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30028591
Proper citation: RRID:Addgene_112255 Copy
Genetic Insert: E2F1RE (responsive element)
Vector Backbone Description: Vector Backbone:pGreenFire1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112248 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
Proper citation: RRID:Addgene_11238 Copy
Species: Homo sapiens
Genetic Insert: TFE3 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562. Use with plasmid pTFE3.1.0-gDNA.
Proper citation: RRID:Addgene_112377 Copy
Species: Homo sapiens
Genetic Insert: TFE3
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTFE3-donor.
Proper citation: RRID:Addgene_112473 Copy
Species: Adenovirus
Genetic Insert: AdML-M3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:pSP72; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215496
Comments: Reed lab clone #388.
Proper citation: RRID:Addgene_11244 Copy
Species: Homo sapiens
Genetic Insert: CUX1
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pCUX1-donor.
Proper citation: RRID:Addgene_112434 Copy
Vector Backbone Description: Vector Backbone:pBAT4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112592 Copy
Species: Synthetic
Genetic Insert: ProD gfpmut3 TT
Vector Backbone Description: Vector Backbone:pFPV25.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29201859
Proper citation: RRID:Addgene_112518 Copy
Vector Backbone Description: Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8660525
Proper citation: RRID:Addgene_112580 Copy
Species: Homo sapiens
Genetic Insert: cathepsin B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339146
Comments: See author's map for more information.
Proper citation: RRID:Addgene_11249 Copy
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