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Species: Synthetic
Genetic Insert: zmiRFP
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108415 Copy
Species: Synthetic
Genetic Insert: xCas9 3.7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108379 Copy
Species: Synthetic
Genetic Insert: beta-Actin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108412 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:939; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108410 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Vector Backbone:pAAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108416 Copy
Species: Synthetic
Genetic Insert: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Comments: Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
Proper citation: RRID:Addgene_108356 Copy
Species: Synthetic
Genetic Insert: B3 recombinase
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pUC57; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Proper citation: RRID:Addgene_108354 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Species: Synthetic
Genetic Insert: Archon2-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108424 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Comments: Addgene QC NGS identified some sequence discrepancies in the plasmid backbone, relative to the depositing lab's provided sequence. Please see the Sequences webpage for additional information.
Proper citation: RRID:Addgene_108423 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108422 Copy
Species: Synthetic
Genetic Insert: dxCas(3.6)-VPR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108388 Copy
Species: Synthetic
Genetic Insert: xCas9(3.6)-ABE(7.10)
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108387 Copy
Species: Synthetic
Genetic Insert: PSalTTC-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108522 Copy
Species: Synthetic
Genetic Insert: TetR + PTet*-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108529 Copy
Species: Synthetic
Genetic Insert: BetIAM + PBetI-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108531 Copy
Species: Synthetic
Genetic Insert: PPhlF-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108512 Copy
Species: Synthetic
Genetic Insert: PBetI-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108519 Copy
Species: Synthetic
Genetic Insert: Archon2-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pHR-EF1a-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108426 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pHR-EF1a-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108425 Copy
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