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Species: Synthetic
Genetic Insert: CRISPRt transposon with gRNA expression cassette (mScar-T03 guide)
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225838 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter H
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225854 Copy
Species: Synthetic
Genetic Insert: CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225853 Copy
Species: Synthetic
Genetic Insert: Tn7 transposon with mScarlet-I expression
Vector Backbone Description: Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, Tn7 Transposition; Bacterial Resistance:Apramycin
Comments: Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint.
Proper citation: RRID:Addgene_225866 Copy
Species: Synthetic
Genetic Insert: AcCas12n
Vector Backbone Description: Vector Backbone:p15a; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:37402371
Proper citation: RRID:Addgene_203806 Copy
Vector Backbone Description: Backbone Size:9558; Vector Backbone:pBAC2015, pGM1190, and pUC19; Vector Types:; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227579 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227581 Copy
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39302127
Comments: Tn7 expression vector.
Proper citation: RRID:Addgene_222354 Copy
Species: Synthetic
Genetic Insert: AmR
Vector Backbone Description: Backbone Size:6370; Vector Backbone:pFNC-6; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227670 Copy
Species: Stackebrandtia nassauensis
Genetic Insert: SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:38947204
Comments: encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225055 Copy
Genetic Insert: apramycin resistance gene
Vector Backbone Description: Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_226171 Copy
Species: Synthetic
Genetic Insert: T7RNAP Circuit
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199993 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233457 Copy
Species: Other
Genetic Insert: Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
Vector Backbone Description: Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41168338
Comments: Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.
Proper citation: RRID:Addgene_233458 Copy
Vector Backbone Description: Vector Backbone:pYH7; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40195982
Comments: Please visit https://doi.org/10.21203/rs.3.rs-6229288/v1 for the article preprint.
Proper citation: RRID:Addgene_240097 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb86; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217503 Copy
Species: Aequorea victoria
Genetic Insert: superfolder green fluorescent protein (sfGFP)
Vector Backbone Description: Vector Backbone:pSEVAb84; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:40471122
Proper citation: RRID:Addgene_217502 Copy
Species: Synthetic
Genetic Insert: Modified Cas9 with polyaspartate linking
Vector Backbone Description: Backbone Size:7510; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:39833194
Comments: Depositor notes that there are SNPs in pSG5 Rep that cause the following amino acid substitutions: L468H R467Q C354W S215A K165E H144R, but these are not of concern for plasmid function.
Proper citation: RRID:Addgene_234428 Copy
Genetic Insert: gRNA targetting desD in Streptomyces
Vector Backbone Description: Backbone Marker:Addgene plasmid #125689 (Weber lab); Backbone Size:12247; Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41942569
Proper citation: RRID:Addgene_219650 Copy
Genetic Insert: Protospacer targeting desD in Streptomyces
Vector Backbone Description: Backbone Marker:Addgene plasmid #125689 (Weber lab); Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:41942569
Proper citation: RRID:Addgene_219651 Copy
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