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  • Bacterial Resistance:apramycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
POSV801-SP44-SnaABCDEOT1T2
 
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RRID:Addgene_225055 SnaA Stackebrandtia nassauensis Apramycin PMID:38947204 encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2 Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin WT 2026-09-05 01:11:41 0
pSuiAb
 
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RRID:Addgene_226171 apramycin resistance gene Apramycin Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:11:42 0
pBroad-T7RNAP-apraR
 
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RRID:Addgene_199993 T7RNAP Circuit Synthetic Apramycin PMID:35366417 Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin copFnull 2026-09-05 01:10:09 0
pCRISPomyces-1
 
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1+ mentions
RRID:Addgene_61736 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-09-05 12:59:05 1
pCRISPomyces-2
 
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10+ mentions
RRID:Addgene_61737 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-09-05 12:59:05 19
pL97
 
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RRID:Addgene_78576 Apramycin Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin 2026-09-05 01:01:44 0
pGM1202
 
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1+ mentions
RRID:Addgene_69615 aac E. coli Apramycin PMID:30191290 Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin 2026-09-05 01:00:16 2
pGM1190
 
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1+ mentions
RRID:Addgene_69994 aac E. coli Apramycin PMID:30191290 Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:00:20 2
pSelAct-Express
 
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RRID:Addgene_174375 Apramycin PMID:34890250 For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning). Original pSelAct backbone was developed here: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 pSelAct-Express was develop and tested here: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin 2026-09-05 01:04:50 0
pEA02
 
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RRID:Addgene_172193 attL Synthetic Apramycin PMID:35456877 Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:04:53 0
pKCcas9dO
 
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1+ mentions
RRID:Addgene_62552 Scocas9 artificially synthesized, codon optimized gene from Strep pyogenes Apramycin PMID:25739462 More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences. Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin 2026-09-05 12:59:12 4
JEC1601
 
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RRID:Addgene_253209 Apramycin PMID:41629321 J23119-Repressor (F3m1)-RNA Primer (-68nt)-Target (F3m1)-J23110-parDE-parCBA-AprR Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:16:05 0
pSW-Apr
 
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RRID:Addgene_252219 SceI Apramycin PMID:36216579 Vector Backbone:pSW-Apr; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:16:10 0
pSuiAb-mltB
 
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RRID:Addgene_253612 mltB Acinetobacter baumannii Apramycin Backbone Marker:Addgene; Vector Backbone:pSuiAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-09-05 01:16:22 0
pTE1643
 
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RRID:Addgene_219650 gRNA targetting desD in Streptomyces Apramycin PMID:41942569 Backbone Marker:Addgene plasmid #125689 (Weber lab); Backbone Size:12247; Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin 2026-09-05 01:16:39 0
pTE1644
 
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1+ mentions
RRID:Addgene_219651 Protospacer targeting desD in Streptomyces Apramycin PMID:41942569 Backbone Marker:Addgene plasmid #125689 (Weber lab); Vector Backbone:pCRISPR-cBEST; Vector Types:CRISPR; Bacterial Resistance:Apramycin 2026-09-05 01:16:39 1
pJMP10745_pCRISPRt-T-apr-T03
 
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RRID:Addgene_225838 CRISPRt transposon with gRNA expression cassette (mScar-T03 guide) Synthetic Apramycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin 2026-09-05 01:17:14 0
pJMP10622_pCRISPRtOE-PH-apr
 
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RRID:Addgene_225854 CRISPRtOE transposon with gRNA expression cassette (no guide) and synthetic promoter H Synthetic Apramycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin 2026-09-05 01:17:14 0
pJMP15019_pCRISPRtOE-noP-apr
 
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RRID:Addgene_225853 CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter Synthetic Apramycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, CRISPR Associated Transposition (CAST); Bacterial Resistance:Apramycin 2026-09-05 01:17:14 0
pJMP10400_pTn7-mscar-apr
 
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RRID:Addgene_225866 Tn7 transposon with mScarlet-I expression Synthetic Apramycin Please visit https://doi.org/10.1101/2024.03.01.582922 for bioRxiv preprint. Vector Backbone:R6K_; Vector Types:Bacterial Expression, Synthetic Biology, Tn7 Transposition; Bacterial Resistance:Apramycin 2026-09-05 01:17:14 0

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