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Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter TATA-C2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14851 Copy
Species: Homo sapiens
Genetic Insert: HsGIGYF1_1-177-YYMLtoAAAA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28698298
Comments: sequence extracted from NCBI: NM_022574.4 or Uniprot.: O75420 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148575 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 helicase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148978 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294208
Comments: HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'.
Proper citation: RRID:Addgene_14858 Copy
Species: Homo sapiens
Genetic Insert: HsNot9_19-285-V181E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28165457
Comments: sequence based on NCBI:NM_005444.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148617 Copy
Species: Drosophila melanogaster
Genetic Insert: DmeIF4G_601-660
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27773676
Comments: ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148506 Copy
Vector Backbone Description: Backbone Size:7107; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.21, Ligation number NONE.
Proper citation: RRID:Addgene_1485 Copy
Vector Backbone Description: Backbone Size:7236; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.57, Ligation number NONE.
Proper citation: RRID:Addgene_1488 Copy
Species: Homo sapiens
Genetic Insert: HsNot1iso1_1351-1588
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28165457
Comments: sequence of isoform 1 given by NCBI: NM_016284.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148630 Copy
Species: Homo sapiens
Genetic Insert: HsNot4_275-575
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30692204
Comments: sequence given by variant 4 NCBI: NM_001190848.1 or Uniprot.: isoform 1 O95628-1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148752 Copy
Species: Homo sapiens
Genetic Insert: STRAD alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA4/HisMax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14985505
Proper citation: RRID:Addgene_14889 Copy
Species: Homo sapiens
Genetic Insert: IgK-IFN
Vector Backbone Description: Backbone Size:5800; Vector Backbone:na; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12356734
Comments: Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.
Proper citation: RRID:Addgene_14886 Copy
Genetic Insert: D kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17189263
Comments: PKD substrate sequence: LSRQLTAAVSE.
Proper citation: RRID:Addgene_14881 Copy
Species: Homo sapiens
Genetic Insert: 3UTR-HLA-A
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148484 Copy
Species: Homo sapiens
Genetic Insert: miR-1
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:2600; Vector Backbone:pTRE-TIGHT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14697198
Comments: Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.
miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).
The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.
Proper citation: RRID:Addgene_14896 Copy
Species: evolved fluorescent protein
Genetic Insert: pmKalama1-Nuc
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-Nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17444659
Proper citation: RRID:Addgene_14894 Copy
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