Plasmids   Select Another Resource Report Type

http://www.addgene.org/15094

Species: Homo sapiens
Genetic Insert: TRAF6 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212
Comments: Mutated version of luciferase reporter construct containing ~500 bp of 3' UTR of TRAF6 with 4-bp substitutions in the miR-146 target sites.

Proper citation: RRID:Addgene_15094 Copy   

•   Source: Addgene

http://www.addgene.org/15091

Species: Homo sapiens
Genetic Insert: miR-146a promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16885212

Proper citation: RRID:Addgene_15091 Copy   

•   Source: Addgene

http://www.addgene.org/15173

Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV Sport 6 with U6 promoter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16426460
Comments: The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system. See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).

Proper citation: RRID:Addgene_15173 Copy   

•   Source: Addgene

http://www.addgene.org/15107

Species: Mus musculus
Genetic Insert: beta2-YFP-M
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Comments: YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M).

Proper citation: RRID:Addgene_15107 Copy   

•   Source: Addgene

http://www.addgene.org/15068

Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TNE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make mRNA for the full length BMP receptor. The clone contains no untranslated sequences.

Proper citation: RRID:Addgene_15068 Copy   

•   Source: Addgene

http://www.addgene.org/15067

Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7522972
Comments: To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.

Proper citation: RRID:Addgene_15067 Copy   

•   Source: Addgene

http://www.addgene.org/15071

Species: Xenopus laevis
Genetic Insert: BMP2-Vg1
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8348610
Comments: PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.). See article for more cloning details. The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.

Proper citation: RRID:Addgene_15071 Copy   

•   Source: Addgene

http://www.addgene.org/140096

Species: Synthetic
Genetic Insert: sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336

Proper citation: RRID:Addgene_140096 Copy   

•   Source: Addgene

http://www.addgene.org/14015

Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.

Proper citation: RRID:Addgene_14015 Copy   

•   Source: Addgene

http://www.addgene.org/140091

Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.

Proper citation: RRID:Addgene_140091 Copy   

•   Source: Addgene

http://www.addgene.org/140312

Species: Homo sapiens
Genetic Insert: Src mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684

Proper citation: RRID:Addgene_140312 Copy   

•   Source: Addgene

http://www.addgene.org/140314

Species: Homo sapiens
Genetic Insert: Src mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684

Proper citation: RRID:Addgene_140314 Copy   

•   Source: Addgene

http://www.addgene.org/140288

Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643

Proper citation: RRID:Addgene_140288 Copy   

•   Source: Addgene

http://www.addgene.org/140206

Vector Backbone Description: Backbone Marker:MIT; Vector Backbone:pLentiCrispr-V2-mOrange (V2mO); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32053639
Comments: Lenti Crispr/Cas9 V2 with mOrange fluorescent marker, to make lentiviral production visible and target cell transduction visible with mOrange. The cloning method for inserting a guide RNA (gRNA) is: BsmB1.

Proper citation: RRID:Addgene_140206 Copy   

•   Source: Addgene

http://www.addgene.org/140296

Species: Homo sapiens
Genetic Insert: Src deletion mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684

Proper citation: RRID:Addgene_140296 Copy   

•   Source: Addgene

http://www.addgene.org/140219

Species: Homo sapiens
Genetic Insert: DNA damage response
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31991288

Proper citation: RRID:Addgene_140219 Copy   

•   Source: Addgene

http://www.addgene.org/140252

Species: Rattus norvegicus
Genetic Insert: eUNG-BE4max(R33A)-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971

Proper citation: RRID:Addgene_140252 Copy   

•   Source: Addgene

http://www.addgene.org/140424

Species: Homo sapiens
Genetic Insert: eGFP-2A-Tau
Vector Backbone Description: Backbone Size:6468; Vector Backbone:AAV.CBA.WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31249873
Comments: Please Note: Plasmid contains a 152bp deletion in the backbone that removes the first 84bp of the ITR upstream of the CAG promoter. This deletion is not known to affect plasmid function. Plasmid 240284 (https://www.addgene.org/240284/) is an alternative version with a complete ITR.

Proper citation: RRID:Addgene_140424 Copy   

•   Source: Addgene

http://www.addgene.org/140552

Species: Homo sapiens
Genetic Insert: 5-HT sensor GRAB_5-HT1.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33821000
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.24.962282v1 for BioRxiv preprint.

Proper citation: RRID:Addgene_140552 Copy   

•   Source: Addgene

http://www.addgene.org/140391

Species: Other
Genetic Insert: T7-lacO-TtgR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301

Proper citation: RRID:Addgene_140391 Copy   

•   Source: Addgene