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Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153232 Copy
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153231 Copy
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153237 Copy
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153236 Copy
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153235 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141301 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141302 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:13299; Vector Backbone:pRATIO1212; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141311 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14000; Vector Backbone:pRATIO2214; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141314 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141299 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141296 Copy
Vector Backbone Description: Backbone Marker:http://shimane-u.org/nakagawa/gbv.htm; Vector Backbone:pGWB505; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209379 Copy
Vector Backbone Description: Backbone Marker:http://shimane-u.org/nakagawa/gbv.htm; Vector Backbone:pGWB506; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209380 Copy
Vector Backbone Description: Backbone Marker:http://shimane-u.org/nakagawa/gbv.htm; Vector Backbone:pGWB505; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209383 Copy
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209389 Copy
Vector Backbone Description: Vector Backbone:pGL2_213; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Contains sfYFP dropout for barcoded-ORI insertion
Proper citation: RRID:Addgene_210697 Copy
Species: Other
Genetic Insert: FAST marker
Vector Backbone Description: Vector Backbone:pJOG272; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:36978193
Proper citation: RRID:Addgene_202775 Copy
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