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Protocol Name
DOI:10.17504/protocols.io.zp8f5rw RRID Copied  
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Malte Kühnemund, Ivan Hernandez, Mohd Istiaq Sharif, Matteo Cornaglia, Martin A.M. Gijs, Mats Nilsson 2019. Detection of bacterial pathogens and drug resistance markers using Rolling circle amplification. protocols.io dx.doi.org/10.17504/protocols.io.zp8f5rw
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.zp8f5rw

Authors: Malte Kühnemund, Ivan Hernandez, Mohd Istiaq Sharif, Matteo Cornaglia, Martin A.M. Gijs, Mats Nilsson

Group: Molecular Diagnostics - Mats Nilsson Group

Summary: Here is described the protocol used to detect bacteria and antibiotic resistance markers using padlock probes and Rolling circle amplification. This method allows for single-molecule/digital quantification in a semi-quantitative way.

Affiliations: Stockholm University, Science for Life Laboratory, Stockholm University, Science for Life Laboratory, Stockholm University, Science for Life Laboratory, EPFL - EPF Lausanne, EPFL - EPF Lausanne, Stockholm University, Science for Life Laboratory

External URL: https://academic.oup.com/nar/article/45/8/e59/2888443

Version: 1

Publication Date: 2019

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Source: Protocols.io