Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Richard Tennant, Paul Rutten 2019. Calibration Protocol - Particle Standard Curve with Microspheres. protocols.io dx.doi.org/10.17504/protocols.io.zgjf3unCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.zgjf3un
Authors: Richard Tennant, Paul Rutten
Group: iGEM Measurement
Summary: You will prepare a dilution series of monodisperse silica microspheres and measure the Abs600 in your plate reader. The size and optical characteristics of these microspheres are similar to cells, and there is a known amount of particles per volume. This measurement will allow you to construct a standard curve of particle concentration which can be used to convert 600 nm absorbance measurements into an estimated equivalent number of cells.
Affiliations: iGEM Measurement Committee, iGEM Measurement Committee
External URL: https://2019.igem.org/Measurement
Version: 1
Publication Date: 2019
Expand AllComing soon.
Check
for all resource mentions.
Coming soon.
Source: Protocols.io
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
