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Harley King 2019. Quantifying Fluorescent Cells in Mammalian Cell Tissue Culture. protocols.io dx.doi.org/10.17504/protocols.io.y2nfydeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.y2nfyde
Authors: Harley King
Summary: Quantifying the change in fluorescent status of cells over time can be an important data point in experimental analysis. Optimally, quantification can be accomplished with flow cytometers. However, these instruments are not available to every lab. Routine analysis and multi-sample preparation can be both time and cost prohibitive.Fluorescent cell quantifcation in cell culture requires a fluorescent microscope and freely available imaging software like Image J.
Affiliations: LuminUltra
Version: 1
Publication Date: 2019
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Source: Protocols.io