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David Eccles 2019. Demultiplexing Nanopore reads with LAST. protocols.io dx.doi.org/10.17504/protocols.io.xj3fkqnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.xj3fkqn
Authors: David Eccles
Group: High molecular weight DNA extraction from all kingdoms
Summary: This protocol is for a semi-manual method for read demultiplexing, as used after my presentation Sequencing DNA with Linux Cores and Nanopores to work out the number of reads captured by different barcodes.Input: reads as a FASTQ file, barcode sequences as a FASTA fileOutput: reads split into single FASTQ files per target [barcode]Note: barcode / adapter sequences are not trimmed by this protocol
Affiliations: Malaghan Institute of Medical Research (NZ)
External URL: https://doi.org/10.5281/zenodo.2535894
Version: 1
Publication Date: 2019
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Source: Protocols.io
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