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Talita Gagliardi 2019. Adapted protocol to extract total RNA using TRIzol® (Invitrogen, Thermo). protocols.io dx.doi.org/10.17504/protocols.io.w8vfhw6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.w8vfhw6
Authors: Talita Gagliardi
Summary: Total RNA extraction using TRizol reagent.Attention: for nasopharyngeal aspirates, we use regular TRizol reagent in this assay, but for blood samples it is better to use a special one as "TRizol LS".
Associated Publications: Matsuno AK, Gagliardi TB, Paula FE, Luna LKS, Jesus BLS, Stein RT, Aragon DC, Carlotti APCP, Arruda E (2019) Human coronavirus alone or in co-infection with rhinovirus C is a risk factor for severe respiratory disease and admission to the pediatric intensive care unit: A one-year study in Southeast Brazil. PLoS ONE 14(6): e0217744. doi: 10.1371/journal.pone.0217744
Affiliations: University of Maryland, College Park
External URL: https://doi.org/10.1371/journal.pone.0217744
Version: 1
Publication Date: 2019
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Source: Protocols.io
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