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Protocol Name
DOI:10.17504/protocols.io.vhde326 RRID Copied  
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Dana Nayduch, Hayley Meier, Christine Mccoy 2018. Feeding bacteria to house flies for microbe fate and gene expression analysis.. protocols.io dx.doi.org/10.17504/protocols.io.vhde326
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.vhde326

Authors: Dana Nayduch, Hayley Meier, Christine Mccoy

Group: ESA MUVE

Summary: Objective: To feed individual house flies a specific amount of bacteria in order to determine bacteria “fate” (persistence, via enumeration; spatiotemporal location, via microscopy) and house fly immune response (whole fly or tissue-specific, via downstream mRNA or protein expression analyses)

Affiliations: USDA-ARS, Arthropod-Borne Animal Diseases Research Unit, USDA-ARS, Arthropod-Borne Animal Diseases Research Unit, USDA-ARS, Arthropod-Borne Animal Diseases Research Unit

Version: 2

Publication Date: 2018

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Source: Protocols.io