Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Katy Monteith 2018. Drosophila Egg Dechorionation. protocols.io dx.doi.org/10.17504/protocols.io.vcze2x6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.vcze2x6
Authors: Katy Monteith
Group: Vale Lab
Summary: This protocol describes how to make an egg dechorionator using available lab equipment before describing how to successfully use the egg dechorionator to remove the outer chorion layer from a Drosophila egg. Egg dechorionation allows cellular analysis, quantitative microinjection and the rearing of developing offspring in a gnotobiotic or axenic environment. This protocol is written with the use of dechorionated eggs for rearing fruit flies in a gnotobiotic or axenic environment in mind. Therefore sterile techniques are described. For cellular analysis and/or quantitative microinjection the protocol can be followed but with a less stringent sterile procedure where unneccesary.
Affiliations: University of Edinburgh
Version: 1
Publication Date: 2018
Expand AllComing soon.
Check
for all resource mentions.
Coming soon.
Source: Protocols.io
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
