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Anna Martinez Casals, Nicholas Mitsios 2019. Sample preparation for single nuclei sequencing of brain. protocols.io dx.doi.org/10.17504/protocols.io.v44e8ywCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.v44e8yw
Authors: Anna Martinez Casals, Nicholas Mitsios
Group: Human Protein Atlas
Summary: Sample preparation for single nuclei sequencing using fresh frozen tissue from human postmortem brain. The protocol is a combination of an already existing and published protocol in Nature protocols Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons and the 10x Genomics handbook Sample preparation, isolation of nuclei for single nuclei RNA sequencing.
Affiliations: Science for life laboratory / KTH - Royal Institute of Technology, Karolinska Institute Stockholm
Version: 1
Publication Date: 2019
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Source: Protocols.io
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