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Protocol Name
16S Illumina Primer Plate Compression
DOI:10.17504/protocols.io.uatesen RRID Copied  
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Rodolfo Salido Benitez 2018. 16S Illumina Primer Plate Compression. protocols.io dx.doi.org/10.17504/protocols.io.uatesen
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.uatesen

Authors: Rodolfo Salido Benitez

Summary: This protocol describes how to make an acoustic droplet ejection compatible plate for liquid handling on the Echo 555 using an epMotion 5075. The protocol expects (4) 96-well primer plates and an empty 384-well low dead volume (LDV) echo plate. The 4 plates will be compressed in a fully interweaved layout. The following images depict this pattern.

Associated Publications: Minich JJ, Humphrey G, Benitez RAS, Sanders J, Swafford A, Allen EE, Knight R, High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity. mSystems 3(6). doi: 10.1128/mSystems.00166-18

Affiliations: Knight Lab @ UC San Diego Health

External URL: https://doi.org/10.1128/mSystems.00166-18

Version: 1

Publication Date: 2018

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Data and Source Information

Source: Protocols.io