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Johannes Debler 2018. Quick Fungal DNA extraction from colonies on plates. protocols.io dx.doi.org/10.17504/protocols.io.snzedf6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.snzedf6
Authors: Johannes Debler
Summary: Adapted from Chi, M. H., Park, S. Y., & Lee, Y. H. (2009).Colony PCR on fungal colonies grown on plates does not work as well as it does for bacteria (it usually doesn't work at all). DNA therefore needs to be extracted first. As this DNA will only be used as a PCR template to check for presence / absence of individual genes I am not too concerend about high molecular weight or purity.This protocol is quick and can be used to process several samples at the same time.
Affiliations: Curtin University
Version: 1
Publication Date: 2018
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Source: Protocols.io
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