Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/10.17504/protocols.io.sjtecnn

Authors: Guangqi Gao, Meng Xu, Yongchun Zuo, Yulan Yang, Chunling Bai, Junyang Xu, Zhuying Wei, Jiumeng Min, Guanghua Su, Xianqiang Zhou, Jun Guo, Yu Hao, Guiping Zhang, Xukui Yang, Xiaomin Xu, Randall B Widelitz, Cheng-Ming Chuong, Chi Zhang, Jun Yin, Guangpeng Li

Group: GigaScience Press

Summary: As one of the most recognizable characteristics in birds, plumage colour has a high impact on understanding the evolution and mechanisms of colouration. Feather and skin are ideal tissues to explore the genomics and complexity of colour patterns in vertebrates. Two species of the genus Chrysolophus, golden pheasant (Chrysolophus pictus) and Lady Amherst’s pheasant (Chrysolophus amherstiae), exhibit brilliant colours in their plumage, but with extreme phenotypic differences, making these two species great models to investigate plumage colouration mechanisms in birds. Here we provide details of the protocols used for plumage pigments identification by High-performance liquid chromatography. The fat-soluble pigments (carotenoids) and oxidation products of melanins (TTCA and PTCA) can be qualitatively and quantificationally analyzed by HPLC with the corresponding standards, respectively.

Affiliations: The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, College of Life Science, Inner Mongolia Agricultural University, Hohhot, 010018, China., College of Life Science, Inner Mongolia Agricultural University, Hohhot, 010018, China., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA., Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA., BGI Genomics, BGI-Shenzhen, Shenzhen 518083, China, College of Life Science, Inner Mongolia Agricultural University, Hohhot, 010018, China., The State key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China.

External URL: www.doi.org/10.5524/100486

Version: 1

Publication Date: 2018

Expand All Collapse