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James Lloyd, Ryan Lister 2018. Extracting plasmid DNA from Agrobacterium with the Bioline ISOLATE II Plasmid Mini Kit. protocols.io dx.doi.org/10.17504/protocols.io.s8fehtnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.s8fehtn
Authors: James Lloyd, Ryan Lister
Summary: To extract plasmid DNA from Agrobacterium, to confirm the correct plasmid has been taken-up, this protocol allows you to directly perform a mini-prep on transformed Agrobacterium, digest this DNA and visualize it on a gel. This is without the need of rescuing the plasmid in E. coli or genotyping with PCR. This protocol using the commercially available Bioline ISOLATE II Plasmid Mini Kit, designed for use with E. coli. The standard protocol this is modified from is available here: https://www.bioline.com/au/downloads/dl/file/id/1219/isolate_ii_plasmid_mini_kit_protocol.pdf
Affiliations: University of Western Australia, University of Western Australia
Version: 1
Publication Date: 2018
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Source: Protocols.io
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