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Protocol Name
DOI:10.17504/protocols.io.rvbd62n RRID Copied  
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Vinay Saunders, David I Wilson 2018. RNA extraction, reverse transcription and PCR for TMSB4X. protocols.io dx.doi.org/10.17504/protocols.io.rvbd62n
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.rvbd62n

Authors: Vinay Saunders, David I Wilson

Summary: Procotol describing RNA extraction from dissected human tissue followed by reverse transcription to cDNA, PCR for detection of TMSB4X and separation of PCR products by agarose gel.

Associated Publications: Saunders V, Dewing JM, Sanchez-Elsner T, Wilson DI (2018) Expression and localisation of thymosin beta-4 in the developing human early fetal heart. PLoS ONE 13(11): e0207248. doi: 10.1371/journal.pone.0207248

Affiliations: University of Southampton, University of Southampton

External URL: https://doi.org/10.1371/journal.pone.0207248

Version: 1

Publication Date: 2018

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Source: Protocols.io