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Erin Cutts, Alessandro Vannini 2018. Troubleshooting biGBac: a practical guide. protocols.io dx.doi.org/10.17504/protocols.io.q3ydypwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.q3ydypw
Authors: Erin Cutts, Alessandro Vannini
Summary: The biGBac system presented by Weissmann et al. (Weissmann et al, 2016 and Weissmann et al, 2018) is a modular insect cell expression system designed for expression of multi-subunit complexes. The system is very powerful, allowing up to 25 genes to be assembled into one baculo-virus expression vector, but does require a reasonable amount of cloning experience in order to attain clones in the 6 day time frame stated in the paper. This guide was written to provide a detailed explanation how the system is designed, how it can be further exploited and provide in-depth practical information that even a novice cloner can follow.
Affiliations: the Institute of Cancer Research, the Institute of Cancer Research
Version: 1
Publication Date: 2018
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Source: Protocols.io
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