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Protocol Name
EMP ITS Illumina Amplicon Protocol
DOI:10.17504/protocols.io.pa7dihn RRID Copied  
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Dylan P. Smith, Kabir G. Peay, Gail Ackermann, Amy Apprill, Markus Bauer, Donna Berg-Lyons, Jason Betley, T. D. Bruns, J. Greg Caporaso, Noah Fierer, Louise Fraser, Jed A. Fuhrman, M. Gardes, Jack A. Gilbert, Niall Gormley, Greg Humphrey, James Huntley, Janet K. Jansson, Rob Knight, Chris L. Lauber, S. Lee, Sarah M. Owens, Alma E. Parada, Geoff Smith, J. Taylor, Luke Thompson, Willam A. Walters, T. J. White 2018. EMP ITS Illumina Amplicon Protocol. protocols.io dx.doi.org/10.17504/protocols.io.pa7dihn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.pa7dihn

Authors: Dylan P. Smith, Kabir G. Peay, Gail Ackermann, Amy Apprill, Markus Bauer, Donna Berg-Lyons, Jason Betley, T. D. Bruns, J. Greg Caporaso, Noah Fierer, Louise Fraser, Jed A. Fuhrman, M. Gardes, Jack A. Gilbert, Niall Gormley, Greg Humphrey, James Huntley, Janet K. Jansson, Rob Knight, Chris L. Lauber, S. Lee, Sarah M. Owens, Alma E. Parada, Geoff Smith, J. Taylor, Luke Thompson, Willam A. Walters, T. J. White

Group: Earth Microbiome Project

Summary: The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP.ITSkabir).

Affiliations: EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium, EMP Consortium

External URL: http://www.earthmicrobiome.org/protocols-and-standards/its/

Version: 1

Publication Date: 2018

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Source: Protocols.io