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Mohd Khubaib 2018. Cell Culture and estimation of cytokines by ELISA. protocols.io dx.doi.org/10.17504/protocols.io.p2hdqb6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.p2hdqb6
Authors: Mohd Khubaib
Summary: this protocol describes the maintainance and treatment of RAW 264.7 macrophage cell lines with Mycobacterial PE/PPE proteins. It also describes estimation of secreted cytokines through ELISA. Estimation of level of IgG subclass from the serum is also described here.
Affiliations: University of Hyderabad
Version: 1
Publication Date: 2018
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Source: Protocols.io
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