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Nicholas Boudreau 2018. Fluorophore Assessment of Glycosidase Activity. protocols.io dx.doi.org/10.17504/protocols.io.nvzde76Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.nvzde76
Authors: Nicholas Boudreau
Summary: Purpose of this protocol is to assess the enzymatic activity of glycosidases using fluorophores conjugated to substrates of interest (such as cellobiose or xylobiose). Once the enzyme of interest cleaves substrate, the fluorophore is released enabling quantification of enzymatic activity.
Affiliations: Dalhousie University
Version: 1
Publication Date: 2018
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Source: Protocols.io