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Yiheng Hu, Benjamin Schwessinger 2018. Amplicon sequencing using MinION optimized from 1D native barcoding genomic DNA. protocols.io dx.doi.org/10.17504/protocols.io.mhkc34wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.mhkc34w
Authors: Yiheng Hu, Benjamin Schwessinger
Group: High molecular weight DNA extraction from all kingdoms, Team Schwessinger
Summary: This is a protocol optimized from Oxford Nanopore Technology 1D native barcoding genomic DNA (with EXP-NBD103 and SQK-LSK108) for sequencing amplicons of fungal marker genes (ITS, EF1a) using PCR.Thanks to Dr. Benjamin Schwesinger for his suggestions about the protocol.Thanks to Dr. Leon Smith (ANU, Linde lab), Prof. Wieland Meyer (USydney) and Dr. Laszlo Irinyi for their contribution of fungal samples.
Affiliations: Australian National University, Australian National University
Version: 1
Publication Date: 2018
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Source: Protocols.io
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