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Vinay K Nagarajan, Monica Accerbi, Pamela J Green 2017. Efforts to transform Heterosigma akashiwo using an Agrobacterium-mediated approach. protocols.io dx.doi.org/10.17504/protocols.io.jsxcnfnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.jsxcnfn
Authors: Vinay K Nagarajan, Monica Accerbi, Pamela J Green
Summary: This protocol was developed for transformation of Heterosigma akashiwo by Agrobacterium tumefaciens. H. akashiwo does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with this protocol and may require further optimization.
Affiliations: Delaware Biotechnology Institute, University of Delaware, Delaware Biotechnology Institute, University of Delaware, Delaware Biotechnology Institute, University of Delaware
Version: 1
Publication Date: 2017
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Source: Protocols.io
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