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Carl De Boer 2017. High-efficiency S. cerevisiae lithium acetate transformation. protocols.io dx.doi.org/10.17504/protocols.io.j4tcqwnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.j4tcqwn
Authors: Carl De Boer
Summary: A protocol for lithium acetate transformation of yeast that can be used to generate highly complex plasmid libraries (O(1E8) if starting with ~1L of culture) and can get over 1 million transformants from a single transformation (as described here). Optimized using yeast strain Y8203. Based on a protocol originally by Supipi Kaluarachchi Duffy.
Affiliations: Broad Institute
Version: 1
Publication Date: 2017
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Source: Protocols.io
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