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URL: https://dx.doi.org/10.17504/protocols.io.hebb3an

Authors: Zita Santos, Patrícia Francisco, Margarida Anjos, Célia Baltazar, Ana Paula Elias, Gabriela Tondolo Fioreze, Pavel M. Itskov, Matthew D. W. Piper, Carlos Ribeiro

Group: Ribeiro Lab

Summary: This is a protocol to generate axenic fly cultures by sterilizing embryos. It is part of the manuscript: Gonçalves et al. Commensal bacteria and essential amino acids control food choice behavior and reproduction. Plos Biology. 2017 Apr 18.The protocol was adapted from:Wayland MT, Defaye A, Rocha J, Jayaram SA, Royet J, Miguel-Aliaga I, et al. Spotting the differences: Probing host/microbiota interactions with a dedicated software tool for the analysis of faecal outputs in Drosophila. J Insect Physiol. 2014;69: 126–135. doi:10.1016/j.jinsphys.2014.05.023with the help of the Leulier laboratory.

Associated Publications: Leitão-Gonçalves R, Carvalho-Santos Z, Francisco AP, Fioreze GT, Anjos M, Baltazar C, Elias AP, Itskov PM, Piper MDW, Ribeiro C, Commensal bacteria and essential amino acids control food choice behavior and reproduction. PLoS Biology 15(4). doi: 10.1371/journal.pbio.2000862

Affiliations: Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences, Champalimaud Centre for the Unknown, School of Biological Sciences

External URL: https://doi.org/10.1371/journal.pbio.2000862

Version: 1

Publication Date: 2017

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