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Lawrence A. Klobutcher 2017. Electroporation of Euplotes crassus: conditions that allow for the uptake of DAPI.. protocols.io dx.doi.org/10.17504/protocols.io.gwmbxc6Copy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.gwmbxc6
Authors: Lawrence A. Klobutcher
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: The electroporation procedure and conditions described allow for the entry of DAPI into the cell, resulting in nuclear staining. We have not yet been successful in using these conditions for transformation of Euplotes crassus, but only a couple of constructs have been tried to date.
Affiliations: University of Connecticut Health Center
Version: 1
Publication Date: 2017
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Source: Protocols.io
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