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Anna Behle 2016. Primer design for assembly-based cloning. protocols.io dx.doi.org/10.17504/protocols.io.gs4bwgwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.gs4bwgw
Authors: Anna Behle
Group: Axmann Lab, CyanoWorld, iGEM Duesseldorf 2018
Summary: This protocol summarizes the process of primer design for assembly-based cloning methods, such as Gibson or AQUA-cloning. Well-designed primers are necessary for efficient cloning of any kind. This protocol can also be used to design primers for overlap extension PCR of linear fragments. Alternatively, overhangs can also include restriction sites for restriction/ligation based cloning.
Affiliations: Institute of Synthetic Microbiology, Heinrich Heine University Düsseldorf
Version: 1
Publication Date: 2016
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Source: Protocols.io
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