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URL: https://dx.doi.org/10.17504/protocols.io.gr6bv9e

Authors: Denville Scientific

Group: Denville Scientific, Inc.

Summary: The SpinSmart PCR purification and gel extraction technologies utilize a lysis buffer containing chaotropic salts that allow DNA to bind to a silica membrane. Binding buffer PCR 1 is added to a PCR reaction or agarose gel slice; the mixture is subsequently loaded directly onto SpinSmart PCR Columns.  Salts, enzymes, and other soluble components are washed away with ethanolic PCR 2 Wash buffer. Purified DNA is eluted using PCR 3 Elution buffer (5 mM Tris/HCl, pH 8.5). Please follow one of the protocols below, depending on whether you are doing a DNA gel extraction or PCR purification.

External URL: https://www.denvillescientific.com/products/spinsmart%E2%84%A2-pcr-purification-gel-extraction-columns-only--with-collection-tubes--50-per-pack

Version: 1

Publication Date: 2016

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