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Protocol Name
Nitrogen-upshift, chemostat to batch mode
DOI:10.17504/protocols.io.ffhbjj6 RRID Copied  
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Darach Miller 2016. Nitrogen-upshift, chemostat to batch mode. protocols.io dx.doi.org/10.17504/protocols.io.ffhbjj6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.ffhbjj6

Authors: Darach Miller

Summary: The aim of this protocol is to describe how to do a very reproducible upshift of a nitrogen-limited yeast culture to nitrogen non-limited conditions. In order to do this with enough cells to make inefficient analysis (ie RATEseq) feasible, we start in the chemostat for high-cell counts.

Version: 1

Publication Date: 2016

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Source: Protocols.io