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URL: https://dx.doi.org/10.17504/protocols.io.f7cbriw

Authors: Oliver Mead

Group: Solomon Lab - Australian National University

Summary: prepare 100 mL per 250 mL flask for each overnight culturefor     CZV8CS - liquid media for flask cultureprepare 100 mL per 250 mL flask for each overnight culture for 100 mL:                                300 mLCzapek-Dox liquid medium         4.54 g                                  13.62 g        centrifuged V8 juice                    20 mL                                   60 mLadd H20 to                                  95 mL                                   285 mL adjust to pH 6.0 transfer totransfer to flask(s) and autoclaveadd 5 mL sterile complete supplement to media after autoclavingComplete Supplement (CS)Bacto-casamino acids     20 g/lBacto-peptone                 20 g/lBacto-yeast extract         20g/lAdenine                           3 g/l 9Biotin                               0.02 g/lNicotinic acid                   0.02 g/lP-aminobenzoic acid       0.02 g/l 10Pyridoxine                        0.02 g/lThiamine                         0.02 g/l

Affiliations: Australian National University

Version: 1

Publication Date: 2016

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