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Protocol Name
Lysate Preparation from Viruses in Mammalian Tissue
DOI:10.17504/protocols.io.f5gbq3w RRID Copied  
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Bioline 2016. Lysate Preparation from Viruses in Mammalian Tissue. protocols.io dx.doi.org/10.17504/protocols.io.f5gbq3w
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.f5gbq3w

Authors: Bioline

Group: Bioline

Summary: The steps for preparing the lysate are different depending on the starting material. Please ensure you follow the correct procedure for your starting material (see the section Disrupting and Homogenizing Starting Materials and the different lysate preparation protocols here). The subsequent steps detailed in Genomic DNA removal and total RNA purification are the same in all cases.This protocol describes the isolation of viral RNA from small amounts of tissue (up to 20 mg for most types of tissue).

External URL: http://www.bioline.com/us/downloads/dl/file/id/3789/isolate_ii_biofluids_rna_kit_product_manual.pdf

Version: 1

Publication Date: 2016

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Source: Protocols.io