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Bioline 2016. Lysate Preparation from Viruses in Mammalian Tissue. protocols.io dx.doi.org/10.17504/protocols.io.f5gbq3wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.f5gbq3w
Authors: Bioline
Group: Bioline
Summary: The steps for preparing the lysate are different depending on the starting material. Please ensure you follow the correct procedure for your starting material (see the section Disrupting and Homogenizing Starting Materials and the different lysate preparation protocols here). The subsequent steps detailed in Genomic DNA removal and total RNA purification are the same in all cases.This protocol describes the isolation of viral RNA from small amounts of tissue (up to 20 mg for most types of tissue).
External URL: http://www.bioline.com/us/downloads/dl/file/id/3789/isolate_ii_biofluids_rna_kit_product_manual.pdf
Version: 1
Publication Date: 2016
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Source: Protocols.io