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Sarah Giuliani 2016. Sampling for RNA / Protein/ dissolved nutrients from phage infection experiments. protocols.io dx.doi.org/10.17504/protocols.io.f27bqhnCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.f27bqhn
Authors: Sarah Giuliani
Group: Coleman Lab
Summary: This sampling protocol is designed to collect two fractions: cell pellets and cell-free filtered supernatants. The cell pellets are used for gene expression analyses by RNA-Seq and proteomics. The cell-free fraction is used for analysis of dissolved analytes (e.g. N, P, DOC). Volumes are based on our experience with cyanobacterial cultures at cell densities ~ 5E7/ml. For more/less concentrated cultures, adjust volumes accordingly.
Affiliations: Coleman Lab - University of Chicago, Department of the Geophysical Sciences
Version: 2
Publication Date: 2016
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Source: Protocols.io
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