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Daniel Vaulot 2015. Fixation of marine samples for flow cytometry sorting. protocols.io dx.doi.org/10.17504/protocols.io.d2x8fmCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.d2x8fm
Authors: Daniel Vaulot
Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff
Summary: Protocol to fix marine samples for flow cytometry sorting of phytoplankton. Fix at least 2 samples per depth sampled and at least 6 to 10 depths per vertical profile.Back to the laboratory, pico and nano-plankton populations can be sorted and used for clone library construction or metabarcoding with Next Generation Sequencing (e.g. Illumina). This was successfully tested on samples from a cruise off Brazil (unpublished data)ReferenceRibeiro C., Lopes A., Marie D., Vaulot D. Unpublished data
Affiliations: Station Biologique, Roscoff, France
Version: 1
Publication Date: 2015
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Source: Protocols.io
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