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Tatiana Karakasheva, Kathryn Hamilton 2021. Isolation of live single cells from intestinal biopsy. protocols.io dx.doi.org/10.17504/protocols.io.bst7nernCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bst7nern
Authors: Tatiana Karakasheva, Kathryn Hamilton
Group: CHOP Gastrointestinal Epithelium Modeling Program
Summary: This protocol describes dissociation of a human intestinal biopsy tissue into single cells, followed by depletion of dead cells via annexin V MACS beads. The outcome is a single-cell suspension with viability ≥ 90% that is used for single-cell sequencing or establishemt of enteroid/colonoid culture.
Affiliations: Children's Hospital of Philadelphia, Gastrointestinal Epithelium Modeling Program, Children's Hospital of Philadelphia Division of Gastroenterology
Version: 2
Publication Date: 2021
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Source: Protocols.io
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