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URL: https://dx.doi.org/10.17504/protocols.io.bst6nere

Authors: Ezequiel G. Mogro, Nicolas Ambrosis, Mauricio Lozano

Summary: Insertion sequences (ISs) are small transposable elements composed only by a transposase and imperfect terminal inverted repeats, which have an important role in genome evolution and contribute to bacterial genome plasticity and adaptability. Bacterial strains from a same species usually present genome rearrangements and variation in the location of insertion sequences and other transposable elements, which might produce phenotypic variations, including antibiotic resistance and adaptation to vaccination strategies. We developed ISCompare to profile IS mobilization events in related bacterial strains. Here we present a comprehensive description on how to use ISCompare, and interpret the obtained results.Basic protocol: Automatic search of Differentially located Insertion Sequences (DLIS)Support protocol 1: Search for differentially located ISs using local filesSupport protocol 2: Using the shift mode to identify differentially located Group II introns

Affiliations: Instituto de Biotecnología y Biología Molecular (IBBM), Instituto de Biotecnología y Biología Molecular (IBBM), Instituto de Biotecnología y Biología Molecular (IBBM)

External URL: https://github.com/maurijlozano/ISCompare

Version: 1

Publication Date: 2021

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