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Shahzad S. Khan, Herschel S. Dhekne, Francesca Tonelli, Suzanne R. Pfeffer 2020. Analysis of Primary Cilia in Rodent Brain By Immunofluorescence Microscopy. protocols.io dx.doi.org/10.17504/protocols.io.bnwimfceCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bnwimfce
Authors: Shahzad S. Khan, Herschel S. Dhekne, Francesca Tonelli, Suzanne R. Pfeffer
Summary: We describe here our method for immunostaining of primary cilia in brain sections from wild type and LRRK2 or other mutant mice. Included are procedures for perfusion, tissue harvesting, sectioning and staining, image acquisition and analysis.
Affiliations: Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307 USA, Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307 USA, MRC Laboratories for Protein Phosphorylation and Ubiquitylation, Dundee, Scotland DD15EH, Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307 USA
Version: 1
Publication Date: 2020
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Source: Protocols.io
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