Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Tim Regan 2020. Preparing biological samples for metabarcoding. protocols.io dx.doi.org/10.17504/protocols.io.bms8k6hwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bms8k6hw
Authors: Tim Regan
Summary: This protocol describes the preparation of biological samples (specifically from a marine environment e.g. hatchery or RAS unit) for amplicon sequencing. Starting with a biological sample stored in Qiagen buffer ATL, or similar, it begins with a bead beating process to homogenise the sample. Enzymatic lysis using Metapolyzyme and Proteinase K are emplyed to ensure efficient DNA release. The Qiagen DNeasy kit is used to column extract DNA from lysates. Following concentration estimates of DNA elutions, samples are diluted >1:10 to avoid PCR inhibition during amplicon library preparation.
Affiliations: The Roslin Institute, University of Edinburgh
Version: 2
Publication Date: 2020
Expand AllComing soon.
Check
for all resource mentions.
Coming soon.
Source: Protocols.io
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
