Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/10.17504/protocols.io.bmmtk46n

Authors: Jeremy Kamil

Summary: I learned this Colloidal Coomassie Blue staining recipe and protocol when I was a postdoc at Harvard Medical School.. Rumor has it, a postdoc reverse engineered a “safe stain” from a leading commercial supplier. It’s a really great recipe: much more sensitive, faster, and easier to use than traditional methanol based coomassie staining protocols you see in Cold Spring Harbor books, like by Maniatis etc.. Saves a ton of money compared to buying this stuff from commercial vendors. It is downstream compatible with mass spec and most other things you'd want to do with gel slices.

Affiliations: Louisiana State University Health Sciences Center Shreveport

Version: 1

Publication Date: 2020

Expand All Collapse