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Benito Recio Totoro 2020. Plasmodium berghei ookinete culture. protocols.io dx.doi.org/10.17504/protocols.io.bmm8k49wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bmm8k49w
Authors: Benito Recio Totoro
Summary: Plasmodium berghei is one of the main non-human malaria models used in research. It readily infects laboratory strains of mice and rats as well as mosquitoes. Because of this, it is safe to manipulate and it does not require human fluids/tissues. This document provides the Operational Procedure for producing and culturing ookinetes of P. berghei in vitro. The method has been optimized for the P. berghei ANKA train clone 2.34 and the derived GFP-expressing clone, but should work for other strains.This method is adapted and modified from:
Affiliations: Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública; Instituto de Biotecnología, Universidad Nacional Autónoma de México
Version: 1
Publication Date: 2020
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Source: Protocols.io
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