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Ken Christensen 2020. SapphireAmp PCR Master Mix -- CHEM 584. protocols.io dx.doi.org/10.17504/protocols.io.bmc7k2znCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bmc7k2zn
Authors: Ken Christensen
Summary: SapphireAmp Fast PCR Master Mix contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent as a 2X premix. SapphireAmp Fast PCR Master Mix is optimized for fast PCR and offers a rapid extension rate (10 sec. per kb). The inclusion of blue dye and a density reagent allows direct loading of PCR products on an agarose gel for electrophoresis. The master mix format simplifies workflows and sample handling; simply add primers, template, and water and then begin PCR. SapphireAmp Fast PCR Master Mix is ideal for fast colony PCR screening. Fast colony PCR amplification of a 5 kb insert can be completed in approximately 1 hr 15 min. Furthermore, it is possible to amplify fragments up to 6 kb from genomic DNA templates.
Affiliations: Brigham Young University
Version: 1
Publication Date: 2020
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Source: Protocols.io
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