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Angel Justiz-Vaillant 2020. Universal Immunoblot analysis for investigating Protein-AG (SpAG)-binding to avian and mammalian immunoglobulins.. protocols.io dx.doi.org/10.17504/protocols.io.bjseknbeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bjseknbe
Authors: Angel Justiz-Vaillant
Summary: A protein that combines the binding capacity of SpA and SpG is not comercially available. It can be easy created in the laboratory by combining these two immunoglobulin-binding proteins to horseradish peroxidase by the periodate method [1]. However, a mixture of SpA and SpG could have the same effect as universal reagent in immunodetection. 1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Affiliations: University of the West Indies St. Augustine
Version: 1
Publication Date: 2020
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Source: Protocols.io
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