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Ye Zheng, Kami Ahmad, Steven Henikoff 2020. CUT&Tag Data Processing and Analysis Tutorial. protocols.io dx.doi.org/10.17504/protocols.io.bjk2kkyeCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bjk2kkye
Authors: Ye Zheng, Kami Ahmad, Steven Henikoff
Summary: This tutorial is designed for processing and analyzing CUT&Tag data following the Benchtop CUT&Tag V.3 protocol. The illustration data used in this tutorial is the profiling of histone modifications in the human lymphoma K562 cell line, but the tutorial is generally applicable to any chromatin protein, including transcription factors, RNA polymerase II, and epitope-tagged proteins. For reproducible analysis, this tutorial is also available on GitHub at https://yezhengstat.github.io/CUTTag_tutorial/.
Associated Publications: Henikoff S, Henikoff JG, Kaya-Okur HS, Ahmad K, Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation. eLife doi: 10.7554/eLife.63274
Affiliations: Fred Hutchinson Cancer Research Center, Fred Hutchinson Cancer Research Center, Fred Hutchinson Cancer Research Center
External URL: https://yezhengstat.github.io/CUTTag_tutorial/
Version: 1
Publication Date: 2020
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Source: Protocols.io
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