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Protocol Name
Zymo Plasmid Miniprep - Classic - CHEM 584
DOI:10.17504/protocols.io.bj5bkq2n RRID Copied  
PDF Report How to cite
Ken Christensen 2020. Zymo Plasmid Miniprep - Classic - CHEM 584. protocols.io dx.doi.org/10.17504/protocols.io.bj5bkq2n
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.bj5bkq2n

Authors: Ken Christensen

Summary: The ZR Plasmid Miniprep-Classic kit is designed for efficient isolation of plasmid DNA from E. coli cell lysates using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast Spin column to yield high-quality plasmid DNA in minutes. The ZR Plasmid Miniprep-Classic features color-coded (red, green, yellow) reagents for easy determination of complete cell lysis. The Zymo-Spin llN columns facilitate high yield plasmid DNA that is endotoxin-free. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

Affiliations: Brigham Young University

Version: 1

Publication Date: 2020

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Data and Source Information

Source: Protocols.io