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Robbie M. Martin, Steven W. Wilhelm 2020. Phenol-based RNA extraction from polycarbonate filters. protocols.io dx.doi.org/10.17504/protocols.io.bivuke6wCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.bivuke6w
Authors: Robbie M. Martin, Steven W. Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: This protocol is an acid-phenol-based method for extracting RNA from samples collected onto polycarbonate filters. Bead-beating is included to increase yields from difficult to lyse cells, for example, in some species of cyanobacteria. We have used it successfully in RNA-sequencing projects involving lab cultures of cyanobacteria and from freshwater and marine environmental samples.
Affiliations: University of Tennessee, Knoxville, University of Tennessee, Knoxville
Version: 1
Publication Date: 2020
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Source: Protocols.io
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