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Protocol Name
Preparing EV-depleted media
DOI:10.17504/protocols.io.biihkcb6 RRID Copied  
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Aizea Morales Kastresana, Bryce Killingsworth, Joshua Welsh, Tim Traynor, Jennifer Jones 2020. Preparing EV-depleted media. protocols.io dx.doi.org/10.17504/protocols.io.biihkcb6
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.biihkcb6

Authors: Aizea Morales Kastresana, Bryce Killingsworth, Joshua Welsh, Tim Traynor, Jennifer Jones

Group: Translational Nanobiology Section

Summary: This protocol describes a method to prepare bovine EV-depleted 10% FBS cell culture media by ultracentrifugation of 20% FBS media and subsequent dilution.

Affiliations: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Translataional Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health

Version: 1

Publication Date: 2020

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Source: Protocols.io